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Finally, to further assess immunogenicity, NetMHC4.1 was used to predict T-cell epitopes and IEDB analysis was used to predict antibody epitopes, in which the REW amino acid substitutions did not significantly increase the number of potentially immunogenic peptides or epitopes compared with WT IgG1 and other Fc-engineering strategies for half-life modulation (Tables S11S12)

Footnotes This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins